Efficient gene transfer into lymphoma cells using adenoviral vectors combined with lipofection
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چکیده
منابع مشابه
Gene Transfer into Sub- cultured Endometrial Cells Using Lipofection
Lipofection using the Lipofectin® reagent was optimized to transiently transfect subcultured guinea pig endometrial stromal cells with a β-galactosidase gene driven by a simian virus 40 promoter. Efficient transfection was obtained in the following conditions: a value of six for the ratio of lipofectin to DNA, a low cellular density (105 cells per 35-mm well) at the time of subculture (48 h bef...
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Lipofection using the Lipofectin reagent was optimized to transiently transfect subcultured guinea pig endometrial stromal cells with a beta-galactosidase gene driven by a simian virus 40 promoter. Efficient transfection was obtained in the following conditions: a value of six for the ratio of lipofectin to DNA, a low cellular density (10(5) cells per 35-mm well) at the time of subculture (48 h...
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The objective of this study was to establish conditions for transfection of a foreign gene into somatic cells using cationic lipid reagents and to evaluate the effects of transfection on in vitro development of somatic cell nuclear transfer (SCNT) embryos. Green fluorescent protein (GFP) gene was used as a foreign gene and a non-transfected somatic cell was utilized as a control karyoplast. Mon...
متن کاملDirect in vivo gene transfer into porcine myocardium using replication-deficient adenoviral vectors.
BACKGROUND Efficient methods of introducing genes into myocardial cells must be developed before local somatic cell gene therapy can be implemented against myocardial disease. Although adenoviral (Ad5) vectors have been used to target rodent hearts and plasmid DNA has been directly injected into the myocardium of rats and dogs, the amounts of recombinant protein produced by these procedures hav...
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The multilayered structure of the cerebral cortex has been studied in detail. Early-born neurons migrate into the inner layer and late-born neurons migrate into more superficial layers, thus establishing an inside-out gradient. The progenitor cells appear to acquire layer-specific properties at the time of neuronal birth; however, the molecular mechanisms of cell-fate acquisition are still uncl...
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ژورنال
عنوان ژورنال: Cancer Gene Therapy
سال: 2000
ISSN: 0929-1903,1476-5500
DOI: 10.1038/sj.cgt.7700209